Chemical reactions in double-stranded nucleic acids. XIV. The efficiency of phosphodiester bond formation between various nucleotide units

I. N. Merenkova, N. G. Dolinnaya, T. S. Oretskaya, N. I. Sokolova, Z. T. Shabarova

Department of Chemistry and A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow

Abstract: Efficiency of the template-directed condensation of oligonucleotides depends on the nature of the nucleotide units to be joined. Fourteen out of sixteen dinucleotide combinations in double-stranded DNA were examined. Alterations of the reaction efficiency are identical for cyanogen bromide and 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide coupling reagents. Dependence of the chemical ligation efficiency on the DNA sequence-specific local conformation is discussed.

Russian Journal of Bioorganic Chemistry 1992, 18 (1):85-91

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