Construction of mRNA-coding DNA templates of preset structure by means of polymerase chain reactions
N. V. Smelkova, A. A. Yolov, Z. A. Shabarova
Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow
Abstract: Combination of chemical DNA synthesis and polymerase chain reactions has been used for obtaining DNA templates of preset structure coding for target mRNAs. The obtained DNA were 136 to 246 bp in length, contained promoters for RNA polymerases of phage T7 or E. coli and coded for 136-membered mRNA.
Russian Journal of Bioorganic Chemistry 1992, 18 (1):78-84