Effects of the physico-chemical factors on the lipoxygenase oxidation of linoleic acid
A. Butovich, E. V. Tsys, T. V. Mogilevich, V. P. Kukhar’
Institute of Bioorganic and Oil Chemistry, Academy of Sciences of the Ukrainian SSR, Kiev
Abstract: Oxidation of linoleic acid by 5-lipoxygenase from potato tubers was studied at different pH-values in the presence of the non-ionic surfactant Lubrol PX, which facilitates UV-monitoring of the process. It is shown that linoleic acid and Lubrol PX form mixed micelles, containing linoleic acid in two major forms with pKal=6.5 (monomeric form localized in the surface layer) and pKa2=8.5 (dimers localized inside hydrophobic core of the micelle). It explains the rise of pH-optimum of the linoleic acid lipoxygenation upon increase of the initial substrate concentration.
Russian Journal of Bioorganic Chemistry 1991, 17 (9):1273-1280