Influence of flanking regions on interleukin-2 chromosome gene expression in mouse myeloma cells
N. N. Anikeeva, T. V. Vinogradova, E. S. Dementyeva, A. R. Ibragimov
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow; All-Union Research Institute of Biotechnology, Moscow; Institute of Molecular Genetics, Academy of Sciences of the USSR, Moscow
Abstract: A human interleukin-2 (IL-2) gene was isolated from genomic DNA library. The isolated gene with 5'- and 3'-flanking sequences of various lengths was unserted into plasmids derived from the retroviral vector pPSneo. The recombinant plasmids were transfected into myeloma X63Ag8-653 cells. The transfected cells, harbouring the IL-2 gene with the shortened (to position –165) or totally deleted 5'-flanking sequence, constitutively expressed biologically active IL-2. Deletion of 3'-flanking region on did not affect the IL-2 expression.
Russian Journal of Bioorganic Chemistry 1991, 17 (9):1223-1228