Isolation and purification of poliovirus 3C protease. Identification of the enzyme's active form
E. P. Sablina, V. K. Antonov
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: All the forms of 3C protease previously found were isolated and purified. A 3D poly-merase's fragment covalently bound with 3C protein does not affect the specific proteolytic activity of the enzyme, whereas the elimination of 26 N-terminal amino acid residues of 3C protease leads to its inactivation.
Russian Journal of Bioorganic Chemistry 1991, 17 (9):1213-1222