Cloning and expression of the poliovirus 3C protease gene in E. coli cells
E. P. Sablina, A. Yu. Amerik, V. K. Antonov
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: Expression of the 3C protease gene of poliovirus type 1 (Mahoney) in E. coli cells using various vectors was studied. The 3C gene was shown to be expressed effectively upon its cloning in HindII/HindII (bases 5240 to 6770) and in HindII/HindIII (bases 5240 to 6056) fragments of poliovirus cDNA in pTTQS vector containing tac-promoter and lacI-repressor gene. Products of processing at the N-terminal 3G protease Gln-Gly site and polypeptides formed upon translation from an alternative methionine, which was coded by bases 5516–5518 of poliovirus cDNA, were found among virus-specific proteins. Processing at the C-terminal 3C protease Gln-Gly site was not observed.
Russian Journal of Bioorganic Chemistry 1991, 17 (9):1201-1212