A rapid deprotection procedure for synthetic oligonucleotides

N. N. Polushin, I. N. Pashkova, V. A. Efimov

M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: A new rapid procedure for the full deprotection of synthetic oligonucleotides has been developed. At 70C, mixture of ethanolamine and ethanol (1:1) removes blocking groups from heterocyclic bases and cleaves the ester linkage between oligonucleotide and solid support within 20 min. Under the same conditions the β-cyanoethyl phosphate protecting group is removed in 2 min methyl phosphate protecting group in 30 min and l-oxido-4-methoxy-6-methyl-2-picolyl group in one hour.

Russian Journal of Bioorganic Chemistry 1991, 17 (8):1145-1148

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