Peroxidase- and laccase-catalyzed removal of phenylhydrazide protecting group under mild conditions

A. N. Semenov, I. V. Lomonosova, V. I. Berezin, M. I. Titov

USSR-Germany Joint Venture .Constanta, Moscow; Science Engineering Co-operative Biotechservis, Moscow

Abstract: A new enzymatic way of removing phenylhydrazide protecting group by the oxido-reductase (peroxidase, laccase) treatment is suggested. The enzymatic oxidation occurs in aqueous solutions at neutral or nearly neutral pH values at room temperature, with the air oxygen or 1 mM hydrogen peroxide as mild oxidizing agents. It was shown that tryptophan, tyrosine and methionine, amino acids most sensitive to oxidation, are not affected during the enzymatic deprotection.

Russian Journal of Bioorganic Chemistry 1991, 17 (8):1074-1076

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