The synthesis of oligonucleotides carrying deoxyuridine C-5-modified with an aliphatic amino group

V. F. Zarytova, N. I. Komarova, A. S. Levina, S. G. Lokhov, D. R. Tabatadze, L.M. Khalimskaya, L.A. Alexandrova

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of the Sciences of the USSR, Novosibirsk; Novosibirsk State University, Novosibirsk; Institute of Molecular Biology, Academy of the Sciences of the USSR, Moscow

Abstract: A method for the synthesis of oligonucleotide derivatives carrying an aliphatic amino group at the C-5-position of deoxyuridine is described. Hexanucleotides dU TCCCA and TdU^RCCCA, where R=CH₂NH₂ or CH₂NHCOCH₂CH₂NH₂ were prepared. The residue of N-(2-hydroxyethyl)phenazinium (Phn) was connected to the amino group of the oligonucleotides. The ability of the obtained hexanucleotides to complementary interaction was tested. It was shown that the stability of the formed duplexes depended on the spacer length.

Russian Journal of Bioorganic Chemistry 1991, 17 (8):1059-1065

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