T4 DNA ligation of 5'-phosphothioate DNA fragments

E. V. Shibanova, D. S. Esipov, E. F. Boldyreva, V. G.Korobko, V. N. Dobrynin

M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: T4 DNA ligation of oligonucleotide 5'-phosphothioate was found to result in the stereospecific formation of the phosphothioate internucleotide linkage with R_p-configuration.

Russian Journal of Bioorganic Chemistry 1991, 17 (8):1048-1050

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