Spectral properties of bisbenzimidasoles interacting with nucleic acids

S. D. Ivanov, A. V. Garabadjiu, N. I. Rtishchev, E. I. Fomina, O. Ju. Kolosova

Central Research Institute of Roentgenology and Radiology, Ministry of Health of the USSR, Leningrad; Leningrad Technological Institute, Leningrad

Abstract: Absorption and fluorescence spectra of various bisbenzimidasole derivatives were studied to estimate the efficiency of their binding to DNA frow salmon testes. The effect of some radicals formed by the phenyl and piperazine rings of the fluorochromes on their interaction with a polynucleotide was determined. It was demonstrated that the Hoechst 33258 fluorescence's increase in presence of rRNA is due to the double-stranded hairpin structures in this polymer.

Russian Journal of Bioorganic Chemistry 1991, 17 (8):1041-1047

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