Nucleotide sequence of an EcoRI-fragment of the rat tyrosine amino- transferase gene determined on the automated sequencer “Applied Biosystems”, model 370A
S. M. Zelenin, V. S. Popova, I. V. Morozov, V. I. Tishkov, A. M. Egorov, N. P. Mertvetsov
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of Science of the USSR, Novosibirsk; A. N. Bach Institute of Biochemistry, Academy of Sciences of the USSR, Moscow; Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow
Abstract: EcoR-I-fragment No. 5 of the rat tyrosine aminotransferase gene containing exons K, L, intron 11, and a part of the 3'-nontranslatable region was digested with several restriction endonucleases (BspR1, Sau3A, BamHI, Ecl136II, Alul), the subfragments obtained were cloned into M13mpl9 and sequenced using the Sanger technique with dye-labelled primers on the automated sequencer “Applied Biosystems”, model 370A. The sequences were combined by means of a PC-GENE package and original programms to yield the primary structure (1064 b. p.) of the above fragment No. 5, adjacent to the previously sequenced EcoRI-fragment No. 4 (3677 b.p.).
Russian Journal of Bioorganic Chemistry 1991, 17 (7):994-996