Synthesis of peptides from the heratitis a virus capsid proteins and their immunochemical study

L. D. Tchikin, A. T. Kozhich, V. S. Ivanov, V. T. Ivanov, T.A. Nastashenko, Yu. Yu. Kusov, M. S. Balayan.

M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow; Institute of Poliomyelitis and Viral Encephalitis, Academy of Medical Sciences of the USSR, Moscow

Abstract: The hepatitis A virus (HAV) capsid protein VP1, VP2 and VPS are exposed at the virion surface and should therefore contain antigenic determinants. Algorithms for hyd-rophilicity, antigenicity and flexibility were used to predict probable antigenic sites. Synthesis of 7- to 23-membered overlapping peptides from seven sites, viz., 111, 117, 233, 1125, 7382, 7686, 98109, 98112, 102107, 102108, 108127, 113123, 118140, 276298 from VP1, 4262 from VP2, 7685 from VPS, and 123 from VP4, was performed by various solid-phase methods.

Free peptides and their conjugates with different carriers were used for immunization and study of antigenicity. The peptides did not interact with antibodies to the hepatitis A virus, whereas their conjugates did not induce the formation of anti-HAV-antibodies.

Russian Journal of Bioorganic Chemistry 1991, 17 (7):964-972

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