Proteins of the rat liver chromation interacting with oligothymidylate derivatives

N. D. Kobetz, E. L. Chernolovskaya, E. M. Ivanova, V. V. Vlassov

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences

of the USSR

Abstract: Upon alkylation of the rat liver chromatin with a hexadecadeoxyribothymidylate derivative bearing 4-(N-2-chloroethyl-N-methylamino)benzylamine residue on the 5'-ter-minal phosphate two nonhiston proteins were modified under conditions of the reagentís forming complementary complexes with poly(A) sequences in DNA. The sequence-specific nature of the reaction is proved by the competition experiments: free oligothymidylate prevented the proteins from alkylation whereas arbitrary oligonucleotide did not. Modification with the reactive oligonucleotide derivatives can be used for the identification of proteins located in the vicinity of the specific open DNA regions in chromatin.

Russian Journal of Bioorganic Chemistry 1991, 17 (7):933-936

Full Text (PDF, in Russian)