Enzymatic synthesis of (β-1,6; 1,3-glucooligosaccha-rides by means of endo-β-1,3-glucanase transglycosilation of gentiooligosaccharides
L. A. Elyakova, V. V. Isakov
Pacific Institute of Bioorganic Chemistry, Far East Division, Academy of Sciences of the USSR, Vladivostok
Abstract: Mixed (β-1,6:1,3-glucooligosaccharides| (d.p. 7–8 or 5–6) were obtained with 15% yield by enzymatic transglycosylation of endo-β-1,3-glucanases from marine mollusks, which transfer β-1,3-bound glucose residues from donors (laminarioligosaccharides) to β-1,6-gentiotetraose as an acceptor. The structures of the formed compounds such as:
G3G3G or G 6 6 G3G G 6 6 G G3G3G 6 6 G G
were proved by 1H and 13C-NMR spectroscopy by the appearance of 1,3,6-bound glucose units in the reaction products. This method is suggested as a new, prompt and one-stage way of synthesis of β-1,6;1,3-glucooligosaccharides, possible inductors of phytoimmunity.
Russian Journal of Bioorganic Chemistry 1991, 17 (6):860-863