Structure of O-specific polysaccharide chain of the Yersinia aldovae lipopolysaccharide
V. A. Zubkov, B. P. Gorshkova, E. L. Nazarenko, A. S. Shashkov, Yu. S. Ovodov
Pacific Institute of Bioorganic Chemistry, Far-East Division,
Academy of Sciences of the USSR, Vladivostok;
N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: O-specific polysaccharide has been isolated on mild hydrolysis of lipopolysaccharide from Yersinia aldovae and shown to consist of 2-acetamido-2-deoxy-D-glucose, D-glucose, 2-acetamido-2-deoxy-D-galactose, and 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-.D-galactose in molar ratio 2:2:1:1. Acid hydrolysis, methylation, solvolysis with anhydrous hydrogen fluoride, 1H and 13C NMR studies indicated the polysaccharide to be composed of hexasaccharide repeating units of the following structure:
Ac | 6 →6)-α-D-GlcNAcp-(1→4)-α-D-GalNAcp-(1→3)-β-D-GlcNAcp-(1→2)-β-D-Glcp-(1→2)-β-D-Fuc3NRp-(1→ 3 ↑ β-Glcp-1 R= —C—CH2—CH—CH3 || | O OH
Russian Journal of Bioorganic Chemistry 1991, 17 (6):831-838