RNA ligase of bacteriophage T4. VIII. A solid-phase enzymic synthesis of oligoribonucleotides
A. V. Mudrakovskaya, V. I. Yamkovoy
Novosibirsk Stale University
Abstract: A method of the solid-phase enzymic synthesis of oligoribonucleotides has been suggested. The donor is fixed through its 3'-end on a water-insoluble matrix followed by the stepwise RNA ligase- and T4 polynucleotide kinase-assisted coupling of trinucleoside diphosphates in the 5'-direction. As an example, (pA)6pAox was immobilised on Biogel P-300 hydrazine and the RNA ligase-catalyzed addition of acceptor ApApA to the donor gave (Ap)9 with the 50% yield.
Russian Journal of Bioorganic Chemistry 1991, 17 (6):819-822