Introduction of aminoalkyl functions into synthesized oligodeoxyribonucleotides via transamination of cytosine residues

N. N. Karpyshev, T. Yu. Bondarenko, I. A. Vtorushina, S. M. Kipriyanov

All-Union Research Institute of Molecular Biology, Koltsovo, Novosibirsk Region

Abstract: The bisulphite-catalysed transamination of cytosine residues by means of ethylenediamine generally used for the natural nucleic acids modification has been extended on relatively short synthetic oligonucleotides. One of the aminoalkyloligonucleotides thus obtained has been used for preparing a biotinylated hybridization probe.

Russian Journal of Bioorganic Chemistry 1991, 17 (6):809-812

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