RNA synthesis by use of T7 RNA polymerase and immobilized DNA in a flowing-type reactor

A. A. Yolov, E. M. Volkov, Z. A. Shabarova

Department of Chemistry, M. V. Lomonosov State University

Abstract: The DNA ligase-induced assembly of synthetic oligodeoxyribonucleotides on polymer supports was used to obtain immobilized DNA, containing the T7 RNA polymerase promoter and coding for a 14-membered oligoribonucleotide. The obtained template can carry out RNA synthesis in a flowing-type reactor. Sepharoso 4B and Toyopear HW-55 were used as supports.

Russian Journal of Bioorganic Chemistry 1991, 17 (6):789-794

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