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Mono- and multilayer membranes with adsorbed glucose oxidase
S. Yu. Zaitsev, Th. Hanke, U. Wollenberger, B. Ebert, N.A. Kalabina, V. P. Zubov, F. Scheller
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow, Central Institute of Molecular Biology, DDR Academy of Sciences, Berlin-Buck
Abstract: Adsorbed layers of glucose oxidase on the air/water interface were prepared and investigated. The transfer of one to ten lipid-protein monolayers onto Pt-electrodes under 20–40 mN/m surface pressure by Langmuir – Blodgett method was performed to yield a biosensor with a measuring range of 0,5–5,0 mM glucose. The amperometric response of the biosensor to glucose depends on the number of the transferred lipid-protein mono-layers and on the surface pressure in the course of the transfer.
Russian Journal of Bioorganic Chemistry 1991, 17 (6):767-772