Localization of the recombinant human interleukin-2 antigenic determinant recognized by the monoclonal antibody 13B1
V. A. Skrivelis, Yu. P. Bundulis, A. V. Panyutich, N. N. Voitenok, V. E. Lunev, V. A. Nesmeyanov, A. Yu. Tsimanis, E. J. Gren
Institute of Molecular Biology, Latvian Academy of Sciences,Riga; Byelorussian Institute of Blood Transfusion and Haematology, Minsk; M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: We suggest a simple approach to localization of antigenic determinants for the monoclonal antibody 13B1 raised against recombinant human interleukin-2. The approach is based on the limited trypsin proteolysis, peptide separation by the O'Farrell method and identification of the peptides, interacting with monoclonal antibodies, and comparison of the charge and length of these peptides with the corresponding values of theoretically possible peptides.
Russian Journal of Bioorganic Chemistry 1991, 17 (6):725-731