The chemical-enzymatic synthesis of the translational enhancer of the phage T7 gene 10 and function of its structural elements

A. I. Gurevich, N. V. Scaptsova, S. V. Lutsenko, V. A. Smirnov, A. N. Kurkin, A. V. Azhayev

M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow; All-Union Research Institute of Biotechnology, Moscow

Abstract: The translational enhancer (TREN) sequence of the phage T7 gene 10 (in full and also its proximal or distal parts) have been obtained by chemical-enzymatic synthesis and cloned into the plasmids immediately before the human interleukin 3 (hIL3) artificial gene. Expression levels of the hIL3 gene in E. coli in these constructions show that the region controlling the specific activity is placed in distal part of TREN more than 40 nucleotides upstream from the initiation codon.

Russian Journal of Bioorganic Chemistry 1991, 17 (5):647-652

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