Enzymatic hydrolysis of glucose and trehalose mycolates, specific glycolipids of mycobacteria and related microorganisms

K. Yu. Gordeev, V. N. Morgunova, G. Ya. Daraseliya, S. G. Batrakov

Research Laboratory of Biologically Active Substances of Hydrobionts, Ministry of Public Health of the USSR, Moscow

Abstract: 6,6'-Di-O-and 6-O-monomycoloyl-α,α-D-trehaloses and 6-O-mycoloyl-D-glucoses, when subjected to hydrolysis with porcine pancreatic lipase (cither soluble or immobilized form), release entirely their mycolic acids in the native state. Therefore, this degradation method has the obvious advantage over acidic and alkaline hydrolyses or alcoholyses and may be useful in studying the structures of glycolipids of the above and similar types. The hydrolysis of trehalose monomycolates is accompanied with the formation of the corresponding dimycolates, which disappear towards the end of the reaction.

Russian Journal of Bioorganic Chemistry 1991, 17 (4):574-575

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