3'-Mercapto-3'-deoxy-TTP terminates DNA synthesis by RNA-dependent DNA polymerases

A. A. Yuzhakov, Z. G. Chidgeavadze, R. Sh. Beabealashvilli, A. A. Krayevsky, G. A. Galegov, M. N. Korneeva, D. N. Nosik, T. Yu. Kilesso

National Cardiology Research Centre, Academy of Medical Sciences of the USSR, Moscow; V. A. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow; D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR, Moscow

Abstract: 3'-Mercapto-3'-deoxy-TTP was synthesized and tested as DNA chain terminator nucleotide for calf thymus alfa DNA polymerase, E. coli DNA polymerase I (Klenow fragment), terminal deoxyribonucleotidyltransferase (Bollum enzyme) and reverse transcriptase from AMV- and HIV-I-viruses. It was shown that the compound terminates DNA chain elongation by reverse transcriptases selectively and irreversibly. Other tested DNA polymerases do not use this nucleotide analogue as a substrate. 3'-Mercapto-3'-deoxythymidine was tested on lymphoblastoid T-cell line MT-4 with HIV-viruses and shown to suppress viruses as efficiently as 3'-azido-3'-deoxythymidine.

Russian Journal of Bioorganic Chemistry 1991, 17 (4):504-509

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