Immunochemical study of surface protein antigenic determinants of tropical malaria parasite plasmodium falciparum with the use of synthetic peptides

S. M. Tertyshnikova, V. V. Yurovski, B. B. Ivanov, V. B. Sadovnikov, A. M. Allakhverdiev

Branch of M. M. Shemyakin Institute of Bioorganic Chemistry, Pushchino, Moscow Region; E. I. Marzinovski Institute of Medical Parazitology and Tropical Medicine, Ministry of Health of the USSR, Moscow

Abstract: To develop new approaches to diagnostics and therapy of malaria, we carried out immunochemical study of the surface proteins of the tropical malaria parasite Plasmodium falciparum with the use of synthetic peptides corresponding to the suggested anti-genic determinants of the parasite proteins. Rabbit antisera raised against the synthetic peptides bound to parasite proteins as shown by ELISA and immunoblotting. Affinity purified anti-peptide antibodies inhibited, in some cases, the parasite growth in the human erythrocytes culture.

Russian Journal of Bioorganic Chemistry 1991, 17 (4):494-503

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