Application of the uracil repair system for extensive deletion of DNA

I. I. Shechter, A. V. Mochulsky, V. P. Veiko, V. G. Debabov

Institute for Genetics and Selection of Industrial Microorganisms, Moscow

Abstract: A procedure for extensive deletion mutagenesis of DNA using the uracil repair system is exemplified by reconstruction of the pBR322 replication regulatory region cloned into M13tgl31. By means of an oligonucleotide primer the 116-nucleotide fragment was excised and four nucleotides were introduced to form a BglII restriction site. Use of the uracil repair selection provided a 30-fold increase in the deletion mutagenesis efficiency.

Russian Journal of Bioorganic Chemistry 1991, 17 (4):456-460

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