Gangliosides from two species of ophiura, Ophiocoma echinata and Ophiomastix annulosa Clark

N. V. Chekareva, G. P. Smirnova, N. K. Kochetkov

N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: The structures of two major and one minor gangliosides from two species of ophiura, Ophiocoma echinata and Ophiomastix annulosa, have been established. On the basis of chemical methods, mass-spectrometry, ¹³C NMR spectroscopy and enzymatic degradation, major gangliosides from two species were identified as NeuGc(α2-6)Glcp(β51-1)Cer and NeuGc8S0₃-(α2-6)Glcp(β1-1)Cer. The minor ganglioside from O. echinata was identified as NeuAc(Gc)(α2-9)NeuAc(α2-6)Glcp(β1-1)Cer. The minor ganglioside from O. annulosa was also found to be disialoganglioside, with the disialyl fragment linked to O6 of the glucose residue of the glucosylceramide moiety. The compositions of the lipid moieties of gangliosides were determined.

Russian Journal of Bioorganic Chemistry 1991, 17 (3):387-397

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