Site-directed mutagenesis with targeting restricted single stranded DNA
I.I. Shechter, K. I. Ratmanova, V. P. Veiko, V. G. Debabov
Institute of Genetics and Selection of Industrial Microorganisms, Moscow
Abstract: Site-directed multiple mutagenesis to obtain hybrides of homologous proteins was carried out by means of the oligonucleotide-targeting digestion of ss DNA. The procedure is more convenient, rapid and simple than the step-by-step] approach. To demonstrate different approaches to targeting digestion of ss DNA, Nco1, Hinf1, Fokl endonucleases were used for DNA cleavage. A hydride of the human and porcine IFN-α2-genes has been constructed.
Russian Journal of Bioorganic Chemistry 1991, 17 (3):379-386