Determination of substrate specificity of a site-specific deoxyribonuclease PtrI

L. R. Lebedev, G. N. Afinogenova, I. S. Andreeva, N. M. Pustoshilova, S. G. Pozdnyakov, V. E. Chizhikov

Scientific Research and Technology Institute of Biologically Active Substances, Berdsk, Novosibirsk Region

Abstract: A new site-specific deoxyribonuclease RtrI from Rhizobium trifolii has been shown to recognize the sequence 5'-G↓TCGAC-3' in double-stranded DNA and to cleave it at the point indicated by an arrow. Therefore the enzyme is a true isoshizomer restriction of the endonuclease SalGI.

Russian Journal of Bioorganic Chemistry 1991, 17 (2):277-279

Full Text (PDF, in Russian)