A temporary protecting group for the synthesis of oligonucleotides containing modified internucleotide linkages
V. V. Samoshin, Yu. E. Khudyakov, V. D. Smirnov
D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR, Moscow
Abstract: Methoxygroup has been used for transient P-protection on H-phosphonate oligonucleotide synthesis. Whereas other H-phosphonate linkages can be irreversibly transformed into phosphoramidates by treatment with alkylamines, the protected phosphoralkoxyl groups generate natural phosphodiester linkages after the final deprotection. The method allows the synthesis of oligonucleotides with addressed position of internucleotide phosphoralkylamidate groups.
Russian Journal of Bioorganic Chemistry 1991, 17 (2):241-245