Genes for human hybrid lymphokines. I. Construction of recombinant plasmids coding for hybrid proteins between human immune interferon and human tumour necrosis factors
V. G. Korobko, I. V. Davydov, L. N. Shingarova, S. A. Filippov, D. S. Esipov, N. P. Berkova, V. N. Dobrynin
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: Recombinant plasmids coding for hybrid proteins between human interferon γ and human tumour necrosis factor α or β have been constructed using site-directed mutagenesis. The genes were fused via a synthetic oligonucleotide linker coding for tetrapeptide Pro-Val-Gly-Pro. The fused genes were expressed in Escherichia coli under control of early promoters of bacteriophage T7. E. coli cells harbouring the plasmids with the hybrid genes gave rather high level of the fused proteins biosynthesis. The hybrid recombinant proteins proved to be unstable in E. coli cells.
Russian Journal of Bioorganic Chemistry 1991, 17 (2):189-196