Synthesis of peptides and localization of the antigenic determinant of the hepatitis B envelope preS1-protein

E. I. Procuronova, I. N. Khlebnikova, G. A. Zheltukhina, R. P. Evstigneeva, Yu. A. Semiletov, V.A. Karpova, T. I. Kalinina, Yu. E. Khudyakov, R. P. Pavluchenkowa, V. D. Smirnov

M. V. Lomonosov Institute of Chemical Technology. Moscow; D I. Ivanovskii Institute of Virology. Academy of Medical Sciences of the USSR. Moscow

Abstract: We synthesized the 2441, 3036, 3136, 2430 fragments of the preS1-region of the hepatitis B (subtype ayw) envelope. The peptides were prepared by the solid phase synthesis on perfluorpolyethylene polymer grafted with polystyrene. The peptide chains were elongated from C-terminus using activated esters and symmetrical anhydrides of Boc-amino acids, cleaved off the solid phase by HBr or TFMSA in TFA, purified by gel filtration, and, after conjugation with protein carriers, inoculated into test animals. The resultant antibodies were shown to react with peptides. The blood sera, from patients with acute hepatitis B reacted with the conjugates of peptides 2441, 3036, 3136 in the immunoenzymic solid phase assay. The monoclonal antibodies-for the preS1-polypeptide were shown to react with peptides 2441, 3036, 3136 and with their conjugates. The results obtained were proved by the data of the epitope mapping with overlapping hexapeptides.

Russian Journal of Bioorganic Chemistry 1991, 17 (11):1500-1509

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