A monoclonal antibody to DNA modified with osmium tetroxide and 2,2'-bipyridine

A. E. Kabakov, L. N. Brovkina, V. K. Podgorodnichenko, A. M. Poverenny

Institute of Medical Radiology, Academy of Medical Sciences of the USSR, Obninsk

Abstract: A monoclonal antibody (IgG) has been produced that binds to DNA modified with-osmium tetroxide and 2,2'-bipyridine and does not react with unmodified DNA and modified or unmodified RNA and proteins. The reaction specificity is due to the presence of the modified deoxythymidine residue within the epitope. Possible use of the antibody for studies of DNA structure and detection of cDNA probes is discussed.

Russian Journal of Bioorganic Chemistry 1991, 17 (10):1386-1389

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