Identification of a site on 18S rRNA of human placenta ribosomes in the region of the mRNA binding centre

D. M. Graifer, M. A. Zenkova, A. A. Malygin, S. V. Mamaev, D. A. Mundus, G. G. Karpova

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences of the USSR

Abstract: The affinity labelling of human placenta 80S ribosomes by 4-(N-2-chloroethyl-N-mothylamino)benzyl-5'-phosphoramjde of hexauridylato has been studied. This mRNA analogue has normal coding properties because its binding to placenta ribosomes significantly increases in the presence of the cognate tRNAPhe. Incubation of the mRNA analogue in the complex with ribosomes and Phe-tRNAPhe leads to its covalent attachment exclusively to the small subunit (mainly to 18S rRNA). The reaction site has been shown by hybridisation experiments to be located within positions 9751055 of 18S rRNA. The identified fragment is located in a highly conserved part of the small subunit rRNA domain II.

Russian Journal of Bioorganic Chemistry 1991, 17 (1):88-98

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