Tick-borne encephalitis virus replicative complex. II. Synthesis of RNA in vitro is effected by antibodies against envelope protein and E protein itself
O. V. Morozova, N. A. Belyavskaya, L. E. Matveev, E. A. Kvetkova, A. G. Pletnev
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of the USSR; Omsk Institute of Natural-Foci Infections, RSFSR Ministry of Health
Abstract: Role of the coat protein E of the tick-borne encephalitis virus in the viral RNA replication in vitro was investigated. It is shown that addition of the exogenous E protein to the TBEV replicative complex inhibits the viral RNA synthesis, whereas addition of polyclonal and monoclonal antibodies against E protein effectively stimulates it. Affinity labelling technique and immunoblot staining of proteins revealed that E protein and anti-E antibodies affect the initiation stage of the viral RNA synthesis.
Russian Journal of Bioorganic Chemistry 1990, 16 (9):1277-1279