Quantitative determination of carbohydrate epitopes of O-antigens from Yersinia pseudotuberculosis and Pseudomonas fluorescens

G. A. Naberezhnykh, T. F. Solov'eva, Yu. S.Ovodov

Pacific Institute of Bioorganic Chemistry, Far East Division, Academy of Sciences of the USSR, Vladivostok

Abstract: Interactions of O-specific polysaccharides, LPS and LPS-protein complex with specific antibodies isolated on a polysaccharide-substituted immunoadsorbent gel and mono-valent Fab-fragments of the antibodies were investigated. The association constants and quantity of Fab-fragments bound to these antigens were established. The interaction of the LPS and LPS-protein complexes was shown to depend considerably on concentration of antigens, and only 1015% of carbohydrate determinants are accessible for binding with antibodies.

Russian Journal of Bioorganic Chemistry 1990, 16 (8):1089-1095

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