Approach to isolation of individual tRNAs from human placenta based on affinity chromatography. Isolation of highly purified Phe-tRNAPhe
V. F. Zarytova, G. G. Karpova, D. A. Mundus, I. G. Shishkina
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences of the USSR
Abstract: A new approach to isolation of individual tRNAs from eukaryotes based on affinity chromatography is suggested. At first, using a sorbent with oligonucleotide pTGGT attached, the total tRNA with native CCA-ends was obtained. Then by means of a sorbent with oligonucleotide pTTCAG immobilized, which is complementary to a part of the tRNAphe anticodon loop, tRNAphe with the acceptor activity>1000 pmole/unit was isolated.
Russian Journal of Bioorganic Chemistry 1990, 16 (8):1069-1072