Anti trans-DDP-DNA antibodies for detection of specific DNA sequences
V. I. Kiseleva, M. F. Turchinsky, A. M. Poverenny
Research Institute of Medical Riadiology, Academy of Medical Sciences of the USSR, Obninsk; M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: DNA modified by trans-diamminedichloroplatinum (trans-DDP) is suggested as an effective probe for non-isotopic hybridisation. Highly specific anti trans-DDP-DNA antibodies and peroxidase-conjugated antibodies to antirabbit Ig and protein A system have been used for the hybrides visualisation. This method allows one to detect 2 pg/mm2 DNA.
Russian Journal of Bioorganic Chemistry 1990, 16 (7):991-992