Efficient manual oligo(poly)deoxyribonucleotide synthesis in pipette tips via H-phosphonate approach
M. G. Isaguliants, V. V. Samoshin, I.V. Makeeva, V. D. Smirnov
D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences, Moscow
Abstract: A facile technique of manual oligonucleotide synthesis via H-phosphonate approach is developed. Syntheses carried out in pipette tips with siliconised glasswool filters take 3–3.5 min per cycle with 97–98% yields per condensation. The method was used to synthesize 12–55-mers: T7 and PL promoter regions, gene of the signal peptide of the E. coli OmpA protein, oligonucleotides coding for amino acid sequences 94–105 of preS1- and 133–143 of preS2-regions of hepatitis B virus, hybridisation probes, sequencing primers, oligonucleolides for site-directed mutagenesis, etc.
Russian Journal of Bioorganic Chemistry 1990, 16 (7):933-940