Some approaches to designing functionally active determinants exemplified by the influenza virus A(H3N2) hemagglutinin
V. A. Shibnev, A. N. Sharetsky, R. V. Valiev, Sh. H. Halilkov
V. A. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow; A.N. Sysin Research Institute of General and Communal Hygiene, Academy of Medical Sciences of the USSR, Moscow; V. I. Lenin Tadjik State University, Dushanbe
Abstract: Hexapeptide Lys-Gly-Pro-Asp-Ser-Gly analogous to the immunodominant fragment 141–146 of the epitop A of the influenza virus A(H3N2) hemagglutinin is synthesized. Conjugated with thyreoglobulin and hemocyanine, the hexapeptide induced formation of highly specific antibodies with heterolitic properties in CBA mice. Antihexapeptide antibodies interact not only with the homologous antigene but also with hemagglutinin and influenza virus. Choice of the hexapeptide sequence is discussed.
Russian Journal of Bioorganic Chemistry 1990, 16 (7):926-932