Isolation and characteristics of new restriction endonucleases from Haemophilus influenzae

L. Lazareviciute, Z. Maneliene, A. Padegimiene, L. Kiuduliene, V. Laucys, J. Bitinaite, I. M. Gruber, V. M. Polyachenko, J. Butkus, A. Janulaitis

All-Union Research Institute of Applied Enzymology, Vilnius: I. I. Mechnikov Central Research Institute of Vaccines and Sera, Academy of Medical Sciences of the USSR, Moscow

Abstract: Various strains of Haemophilus influenzas have been examined for the presence of site-specific endonuclease activities, and eleven restriction endonucleaseshave been isolated from seven strains. For all the endonucleases recognition sequences were determined, for three of them cleavage sites being identified. The enzymes proved to be isoschizomers of known endonucleases, viz. Hini I, Hin8 I — Acy I; Hini II, Hin8 II—Nla III; Hin2 I, Hin5 I — Hpa II; Hin3 I — Cau II; Hin5 II — Asu I; Hin5 III — Hind III; Hin6 I, Hin7 I — Hha I. Restriction endonucleases Hin1 I, Hin1 II and Hin6 I recognize nucleotide sequences 5'GRCGPYC, 5'CATG, 5'GCGC, respectively, and cleave them as indicated by arrows.

Russian Journal of Bioorganic Chemistry 1990, 16 (7):889-897

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