The structure of carbohydrate chains of hemagglutinin and neuraminidase of influenza virus B/Leningrad/179/86

N. P. Arbatsky, A. O. Zheltova, D. V. Yurtov, I. G. Kharitonenkov, Yu. P. Abashev, V. A. Derevitskaya, N. K. Kochetkov

N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow: D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR, Moscow

Abstract: The main surface glycoprotein, hemagglutinin (HA), was obtained by treatment of influenza virus B/Leningrad/179/86 with bromelain. Amino acid and monosaccharide compositions of HA and neuraminidaso (NA, earlier isolated from the same virus) were determined, thus showing HA and NA to contain 810 and 2 carbohydrate chains, respectively The carbohydrate fragments were cleaved off by the alkaline LiBH4 treatment, the oligosaccharides released were reduced with NaB3H4 and fractionated by two-step HPLC on Ultrasphere-C18 and Zorbax-NH2 columns. Some higher mannose and complex oligosaccharides were identified in both cases by comparison with nonla-belled oligosaccharides of the known structure. The data obtained show that surface glycoproteins of influenza virus A and B are rather similar with regard to structure and heterogeneity of their carbohydrate chains.

Russian Journal of Bioorganic Chemistry 1990, 16 (6):801-807

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