Reconstruction of a synthetic human interleukin-2 gene

S. V. Seryogin, V. A. Ryabinin, A. N. Sinyakov, N. K. Danilyuk, S. G. Pozdnyakov

All-Union. Research Institute of Molecular Biology, Kolítsovo, Novosibirsk Region

Abstract: Chemical-enzymatic synthesis and cloning of the DNA fragment coding for the human interleukin-2 signal sequence was accomplished. A hybrid plasmid pSIL-2 containing the gene of the human interleukin-2 with this signal sequence was constructed for effective expression of the gene in eukaryotic systems. A variant permitting the removal of the interleukin-2 stop-codons was obtained, which is suitable for the construction of chimeric genes containing the interleukin-2 gene sequence at the 5'-end.

Russian Journal of Bioorganic Chemistry 1990, 16 (6):759-764

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