Ligand-exchange chromatography of proteins and enzymes

S. A. Lopatin, V. P. Varlamov, V. A. Davankov

A. N. Nesmeyanov Institute of Organoelement Compounds, Academy of Sciences of the USSR, Moscow

Abstract: Current state of the ligand-exchange chromatography (metal chelate affinity chro-matography) of proteins and enzymes is reviewed. This technique is based on the ability of proteins to bind metal ions immobilized on chelate gels. The influence of pH, composition of buffer, type of stationary ligand and nature of metal ions on the chromatographic behaviour of proteins is discussed.

Russian Journal of Bioorganic Chemistry 1990, 16 (6):725-750

Full Text (PDF, in Russian)