3 H NMR analysis of tritium- labelled glycine and alanine
S. G. Rosenberg, V. M. Zaloznykh, Yu.A. Zolotarev, V. S. Kozik, N. F. Myasoedov
Institute of Pharmacology of the USSR, Moscow; Institute of Geochemistry and Analytical Chemistry, Academy of Sciences of the USSR, Moscow; Institute of Molecular Genetics, Academy of Sciences of the USSR, Moscow
Abstract: A method for analysis of three-component isotopic mixtures of tritium-labelled gly-cine and alanine in D2O solutions has been developed on the basis of high resolution 3H NMR spectra at 266.8 MHz. Determined were composition of the mixtures in molar per cent, as well as geminal and vicinal coupling constants (2 J 3H,H Gly=-16,4±0,2 Hz; 2JAla 3H,H=-14,0±0,5 Hz; 3JAla 3H,H=7,6±0,2 Hz) and isotopic shifts (0,021±0,001 ppm for glycine; 0,026±0,001 ppm for alanine).
Russian Journal of Bioorganic Chemistry 1990, 16 (5):715-717