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Replacement of Water by Water-Organic Mixture in Reversed Mice Liar Systems as a Way to Increase the Efficiency of Enzymatic Catalysis
N. L. Klyachko, N. G. Bogdanova, K. Martinek, A. V. Levashov
Department of Chemistry, M. V. Lomonosov Moscow State University, Moskow
Institute of Organic Chemistry and Biochemistry, Czechoslovakian Academy of Sciences, Prague, CSSR
Abstract: Catalytic properties of α-chymotrypsin, peroxidase, and laccase dissolved in the system of surfactant reversed micelles solvated by aqueous glycerol, butanediols, and dimethylsulfoxide were studied. As micelle-forming surfactants, sodium salt of di-2-et-hylhexyl sulfosuccinate (Aerosol OT) in octane and cetyltrimethylammonium bromide in octane – chloroform (1:1) mixture were used. The dependence of catalytic activity on the surfactant solvation degree was bell-shaped. Optima of catalytic activity of enzymes solubilized in such systems were observed when diameters of micellar inner cavity and of the entrapped protein molecule were equal. With decreasing water content on water-organic mixtures (down to 5 vol. %) the level of the optimal catalytic activity of the •enzymes increased considerably.
Russian Journal of Bioorganic Chemistry 1990, 16 (5):581-589