COMPARATIVE INHIBITION ANALYSIS OF DNA BIOSYNTHESIS CATALYZED BY RETROVIRUS REVERTASES
T. G. KILESSO, N. B. TARUSOVA *, E. D. ATRAZHEVA*, M. K. KUKHANOVA *, S. V. SHULENIN, A. Ph. BOBKOV, M. M. GARAEV, G. A. GALEGOV, A. A. KRAYEVSKY*
D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR, Moscow; * V. A. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow
Abstract: Comparative study of DNA biosynthesis inhibition, catalyzed by avian myeloblastose virus (AMV) reverse transcriptase (RT), human immunodeficiency virus (HIV) recombinant and native RT, has been performed. 3'-Azido-2',3'-dideoxy-thymidine 5'-triphosphate (AzTTP); 3'-azido-2',3'-dideoxythymidine 5'-methylenepho-sphonate-diphosphate; 3'-azido-2' ,3'-dideoxythymidine 5'-phosphate-phospono-acetate; 3'-azido-2',3'-dideoxythymidine 5'-phosphate-dibromomethylenephosphonate; 2',3'-O-isopropylidenecytidine 5'-methylenephosphonate-diphosphate (rC-iP-MPDP) were used as inhibitors. AzTTP proved to by the most active inhibitor (its activity against HIV RT is higher than against AMV RT), although not selective as the phosphonates; only rC-iP-MPDP has low selectivity.
Russian Journal of Bioorganic Chemistry 1990, 16 (4):531-536