THE RNA CLEAVAGE BY HYBRIDASE. I. OLIGONUCLEOTIDE PROBES FOR REGIOSPECIFIC RNA CLEAVAGE
V. G. METELEV, N. F. KRYNETSKAYA, A. A. PURMAL, Z. A. SHABAROVA
A. N. Belozersky Laboratory and Chemical Department, M. V. Lomonosov Moscow State University
Abstract: New oligonucleotide probes for regiospecific cleavage of RNA molecules by hybri-. dase (RNase H) are suggested. RNase H from E.coli is shown to site-specifically split eight phosphodiester bonds in RNA in the heteroduplex, formed by 5S rRNA and d(ACCACCGCGCT). The partial substitution of deoxycytidines in position 5, 6, 8, 10 of the probe by 2'-O-methylcytidines leads to unique (regiospecific) RNA cleavage between U25 and C26.
Russian Journal of Bioorganic Chemistry 1990, 16 (4):507-513