ISOLATION AND PRIMARY CHARACTERIZATION OF THE COMMON SPECIES-SPECIFIC OUTER MEMBRANE PROTEIN OF RICKETTSIA PROWAZEKII
A. V. RODIONOV, L. S. BELOUSOVA, I. P. ISACHKOVA, G. N. PLUZNIKOVA, B. A. DMITRIEV, E. V. MOLOKOVA
N. F. Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences of the USSR, Moscow
Abstract: Common species-specific protein is isolated for the first time in the chromatographi-cally pure state from the outer membrane of Ricketsia prowazekii , and its amino acid composition is determined. As revealed by chromatofocusing technique, the protein possesses pI 4.18±0.,03. Three basic ninhydrine-positive compounds, differing from usual amino acids, were discovered in the, protein hydrolyzate. Data suggesting the subunit structure of the isolated protein are presented.
Russian Journal of Bioorganic Chemistry 1990, 16 (4):457-463