THE METABOLIZAT10N OF EXOGENOUS [1- 14 C ]CORONARIC AND [1- 14 C]VERNOLIC ACIDS IN HOMOGENATE OF PEA SEEDLINGS

A. N. GRECHKIN, N. V. KUKHTINA, H. A. KURAMSHIN, E. Y. SAFONOVA, Y.J.YEFREMOV *, I. A. TARCHEVSKY

Kazan Institute of Biology: * A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Branch of Academy of Sciences of the USSR

Abstract: Metabolization of (Z)-[1-14Cl-9,10-epoxy-12-octadecenoic (coronaric) and (Z)-[1-14C]-12,13-epoxy-9-octadecenoic (vernolic) acids in homogenate of epicotyls of pea seedlings was studied. After purification by HPLC, metabolites were identified by high resolution mass spectrometry as (9, 10), (12, 13)-diepoxyoctadecanoic (I); (Z)-9,10-dihydroxy-12-octadecenoic (II); (Z)-12,13-dihydroxy-9-octadecenoic (III); 9,10-dihydroxy-12,13-epoxy-octadecanoic (IV) and 9,10-diepoxy-12,13-dihydroxyoctadecanoic (V) acids. The label from [1-14C]coronaric acid was incorporated into compounds (I), (II) and (IV), and from [1-14C]vernolic acid into metabolites (I), (III) and (V). Compounds (I), (IV) and (V) have not been previously found in natural sources. The pathways of formation of acids (I) (V) and their possible role in the endogenous regulation of plants are discusses

Russian Journal of Bioorganic Chemistry 1990, 16 (3):413-418

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